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Tree Physiology Advance Access originally published online on December 23, 2008
Tree Physiology 2009 29(2):291-298; doi:10.1093/treephys/tpn027
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© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Influences of aging and cloning methods on the capacity for somatic embryogenesis of a mature Hevea brasiliensis genotype

Ludovic Lardet1,2, Florence Dessailly1, Marc-Philippe Carron1, Pascal Montoro1 and Olivier Monteuuis1

1 CIRAD-BIOS, UMR 1098 DAP, BURST Research Unit TA A-96/03, Avenue Agropolis, 34398 Montpellier Cedex 5, France
2 Corresponding author (ludovic.lardet{at}cirad.fr)


  Abstract

We compared embryogenic capacities of integument explants excised from three sources of the Hevea brasiliensis (Müll. Arg.) mature genotype PB 260. The three sources were 17-year-old (BT 86) and 7-year-old (BT 96) budded trees and 7-year-old emblings (EM 96). The highest proportions of embryogenic calluses obtained from the total number of integument explants initially used were from trees of EM 96 origin, followed by BT 96 trees, with explants from BT 86 trees producing the lowest number of embryogenic calluses. Further initiation of embryogenic callus lines from the primary somatic embryos derived from the three sources was successful only for EM 96. Somatic embryo cultures from BT 86 and BT 96 sources produced only friable calluses that could not be further amplified. Overall, somatic embryo explants derived from EM 96 responded over a wider range of 3,4-dichlorophenoxyacetic acid and kinetin concentrations than the somatic embryo explants from BT 86 and BT 96 origins. The effects of chronologic, ontogenetic and physiologic aging on explant capacity for somatic embryogenesis and on the overall efficiency of the process in H. brasiliensis are discussed.

Keywords: callogenesis, maturation, ontogenesis, phase change

Received February 4, 2008; Accepted September 30, 2008


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