Tree Physiology Advance Access published online on October 27, 2009
Tree Physiology, doi:10.1093/treephys/tpp092
Transient thermal dissipation method of xylem sap flow measurement: multi-species calibration and field evaluation
1 Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002 Thailand
2 UMR547 PIAF, INRA, Université Blaise Pascal, 63100 Clermont-Ferrand, France
3 Institute of Research for Development (IRD), Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
4 Corresponding author (frederic.do{at}ird.fr)
5 Institute of Research for Development (IRD), Land Development Department, Bangkok, Thailand
6 Institute of Research for Development (IRD), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Montpellier, France
 |
Abstract |
|---|
The transient thermal dissipation (TTD) method developed by Do and Rocheteau (2002b) is a close evolution of the original constant thermal dissipation (CTD) method of Granier (1985). The TTD method has the advantage of limiting the influence of passive natural temperature gradients and of yielding more stable zero-flux references at night. By analogy with the CTD method, the transient method was first calibrated on synthetic porous material (sawdust) on the assumption that the relationship was independent of the woody species. Here, our concern was to test the latter hypothesis with a 10-min heating time in three tropical species: Hevea brasiliensis Müll. Arg., Mangifera indica L. and Citrus maxima Merr. A complementary objective was to compare the field estimates of daily transpiration for mature rubber trees with estimates based on a simplified soil water balance in the dry season. The calibration experiments were carried out in the laboratory on cut stems using an HPFM device and gravimetric control of water flow up to 5 L dm–2 h–1. Nineteen response curves were assessed on fully conductive xylem, combining 11 cut stems and two probes. The field evaluation comprised five periods from November 2007 to February 2008. Estimates of daily transpiration from the measurement of sap flow were based on the 41 sensors set up on 11 trees. Soil water depletion was monitored by neutron probe and 12 access tubes to a depth of 1.8 m. The calibrations confirmed that the response of the transient thermal index to flow density was independent of the woody species that were tested. The best fit was a simple linear response (R2 = 0.88, n = 276 and P < 0.0001). The previous calibration performed by Do and Rocheteau (2002b) on sawdust fell within the variability of the multi-species calibration; however, there were substantial differences with the average curve at extreme flow rates. Field comparison with soil water depletion in the dry season validated to a reasonable extent the absolute estimates of transpiration acquired with the 10-min TTD method. In conclusion, evidence for the independence of calibration from woody species and the simple linear response of the thermal index strengthen the interest of the TTD method with 10-min heating.
Keywords: Citrus maxima, cut stem experiment, Granier’s sensors, Hevea brasiliensis, Mangifera indica, soil water balance, tree transpiration, validation
Received June 17, 2009; Accepted September 28, 2009