Tree Physiology Advance Access originally published online on July 16, 2009
Tree Physiology 2009 29(9):1143-1151; doi:10.1093/treephys/tpp051
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The influence of nitrogen and phosphorus supply and genotype on mesophyll conductance limitations to photosynthesis in Pinus radiata
1 Faculty of Forestry, University of Chile, P.O. Box 9206, Santiago, Chile
2 Corresponding author (hbown{at}uchile.cl)
3 Scion, P.O. Box 29237, Christchurch, New Zealand
4 School of Forestry, University of Canterbury, Private Bag 4800, Christchurch, New Zealand
5 Landcare Research, P.O. Box 40, Lincoln 7640, Christchurch, New Zealand
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Abstract |
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Mesophyll conductance, gm, may pose significant limitations to photosynthesis and may be differentially affected by nutrition and genotype in Pinus radiata D. Don. Simultaneous measurements of gas exchange and chlorophyll fluorescence were made to determine gm, using the constant J method (Harley, P.C., F. Loreto, G. Di Marco and T.D. Sharkey. 1992. Theoretical considerations when estimating the mesophyll conductance to CO2 flux by analysis of the response of photosynthesis to CO2. Plant Physiol. 98:1429–1436), in a fast- and a slow-growing clone of P. radiata grown in a greenhouse with a factorial combination of nitrogen (N) and phosphorus (P) supply. Values of gm increased linearly with the rate of photosynthesis at saturating irradiance and ambient CO2 concentration, Asat (gm = 0.020Asat, r2 = 0.25, P < 0.001) and with stomatal conductance to CO2 transfer, gs (gm = 1.16gs, r2 = 0.14, P < 0.001). Values of gm were greater than those of stomatal conductance, gs, and the ratio (gm/gs) was not influenced by single or combined N and P additions or clone with a mean (±SE) value of 1.22 ± 0.06. Relative limitations to mesophyll conductance, Lm (16%) to photosynthesis, were generally greater than those imposed by stomata, Ls (13%). The mean (±SE) CO2 concentration in the intercellular air spaces (Ci) was 53 ± 3 µmol mol–1 lower than that in the atmosphere (Ca). Mean (±SE) CO2 concentration in the chloroplasts (Cc) was 48 ± 2 µmol mol–1 lower than Ci. Values of Ls, Lm and CO2 diffusion gradients posed by gs (Ca – Ci) and gm (Ci – Cc) did not significantly differ with nutrient supply or clone. Mean values of Vcmax and Jmax calculated on a Cc basis were 15.4% and 3.1% greater than those calculated on a Ci basis, which translated into different slopes of the Jmax/Vcmax relationship (Cc basis: Jmax = 2.11Vcmax, r2 = 0.88, P < 0.001; Ci basis: Jmax = 2.43Vcmax, r2 = 0.86, P < 0.001). These results will be useful for correcting estimates of Vcmax and Jmax used to characterize the biochemical properties of photosynthesis for P. radiata.
Keywords: chloroplastic CO2 concentration, electron transport, nutrient limitation, Rubisco carboxylation, stomatal limitation
Received May 12, 2009; Accepted June 25, 2009