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Tree Physiology Advance Access originally published online on January 13, 2009
Tree Physiology 2009 29(3):411-421; doi:10.1093/treephys/tpn034
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© The Author 2009. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine

Hanna Shin1,3, Hyoshin Lee1,2, Kwan-Soo Woo1, Eun-Woon Noh1, Yeong-Bon Koo1 and Kyung-Joon Lee3

1 Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea
2 Corresponding author (hslee{at}forest.go.kr)
3 School of Forest Sciences, Seoul National University, Seoul 151-921, Korea


  Abstract

Pine wilt disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle, has destroyed huge areas of pine forest in East Asia, including Japan, China and Korea. No protection against PWN has been developed, and the responses of pine trees at the molecular level are unrecorded. We isolated and analyzed upregulated or newly induced genes from PWN-inoculated Japanese red pine (Pinus densiflora Sieb. et Zucc.) by using an annealing control primer system and suppression subtractive hybridization. Significant changes occurred in the transcript abundance of genes with functions related to defense, secondary metabolism and transcription, as the disease progressed. Other gene transcripts encoding pathogenesis-related proteins, pinosylvin synthases and metallothioneins were also more abundant in PWN-inoculated trees than in non-inoculated trees. Our report provides fundamental information on the molecular mechanisms controlling the biochemical and physiological responses of Japanese red pine trees to PWN invasion.

Keywords: annealing control primer system, differentially expressed genes, pine wilt disease, Pinus densiflora, suppression subtractive hybridization

Received July 27, 2008; Accepted November 7, 2008


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